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Research Interest

My background in Molecular Microbiology and Food Science allowed me to work with some of the important pathogenic bacteria in food and environment. Currently, our laboratory (Microbiology Lab) is known for working on many different pathogenic species such as Vibrio parahaemolyticus, Vibrio cholerae, Vibrio vulnificus, Escherichia coli O157:H7, Listeria monocytogenes and Salmonella species. Our works encompass the isolation and characterization of the pathogens both by conventional as well as molecular methods from food and environment. The following details of the previous or on-going researches and selected publications are simply a reflection of our involvement in the food safety as well as other microbiological research in collaboration with local research network and international.

Current research topics:

Isolation and characterization (conventional and molecular methods) of Vibrio spp. in seafood, raw vegetables, meats and street food in Kuching, Sarawak.

Bacterial enteropathogens isolated from migratory birds and rodents in different ecological habitats in Sarawak, Malaysia.

Antibiotic resistance of bacteria in aquacultural environments in Sarawak.

Predicting Transmission Pathway of Enterobacteria from Wildlife Hosts to Environment in Sarawak, Malaysia.

Isolation, identification and characterization of anaerobic ammonium-oxidizing (anammox) bacteria in waste water treatment plant in Kuching, Sarawak.

Distribution of E. coli, Campylobacter, Salmonella and Listeria monocytogenes in raw vegetables in Sarawak.

Publication

Lesley Maurice Bilung, Velnetti Linang, Cheah Yoke Kqueen, Son Radu, Kasing Apun, Samuel Lihan, Micky Vincent and Mitsuaki Nishibuchi. (2011) Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) at Tanjung Karang, Kuala Selangor. Submitted to International Food Research Journal. Manuscript ID: IFRJ-2011-012.

Kho Kai Ling, Kasing Apun, Lesley Maurice Bilung, Mohd. Tajuddin Abdullah, Mustafa Abdul Rahman, Chen Yik Ming, Adom Benjamen, Chong Yee Ling, and Hashimatul Fatma Hashim, Occurrence of Escherichia coli in wildlife from different habitats of Sarawak, Malaysia. (2010) (Submitted to Borneo Journal of Resource Science and Technology)

Kasing Apun, Kho Kai Ling, Chong Yee Ling, Hashimatul Fatma Hashim, Mohd. Tajuddin Abdullah, Mustafa Abdul Rahman, Lesley Maurice Bilung and Samuel Lihan (2011) Detection of Escherichia coli O157:H7 in wildlife from disturb habitats in Sarawak. Research Journal of Microbiology, 6, 132-139. 

Tunung, R., Ghazali, F.M., Noranizan, M.A., Haresh, K.K., Lesley, M.B., Nakaguchi, Y., Nishibuchi, M. and Son, R. (2011) Rapid detection and enumeration of pathogenic Vibrio parahaemolyticus in raw vegetables from retail outlets. International Food Research Journal 18: 67-78.

Zulkifli, Y., Alitheen, N. B., Son, R., Yeap, S. K., Lesley, M. B. and Raha, A. R. (2009) Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes. International Food Research Journal 16: 289-296.

Chandrika Murugaiah, Zainon Mohd Noor, Maimunah Mastakim, Lesley Maurice Bilung, Jinap Selamat and Son Radu. (2009) Meat species identification and Halal authentication analysis 3 using mitochondrial DNA. Meat Science. 83(1):57-61.

R. Tunung, L.C. Chai, M.R. Usha, M.B. Lesley, Y.K. Cheah, G.B. Patrick, M.G. Farinazleen, A.B. Fatimah, P.K. Malakar and R. Son. (2007) Incidence and characterization of Salmonella species in street food and clinical samples. Journal of Food Safety 27: 345-361.

Ooi, W. L., Haryiani, Y., Lesley, M. B., Tunung, R., Jurin, W. G., Michael, C. W. V. L. and Son Radu, Vancomycin resistance Enterococci (VRE) in poultry, vegetable, environmental and clinical sources. 2006 Malaysian Applied Biology 35: 49-56.

Lesley, M. B., Son Radu, Bahaman, A. R., Raha, A. R., Napis, S., Michael, W. C. V. L., Gwendelynne, B. T. and Nishibuchi, M. Detection of Vibrio parahaemolyticus in cockle (Anadara granosa) by using specific-PCR. 2005. FEMS Microbiology Letters 25: 85-88.

Gwendelynne, B. T., Son Radu, Nishibuchi, M.,  Raha, A. R., Napis, S., Lesley, M. and Jurin, W. G. Characterization of Vibrio parahaemolyticus isolated from coastal seawater in Peninsular Malaysia. 2005. Southeast Asian Journal of Tropical Medicine and Public Health 36: 940-945.

Jacintha Sugnaseelan, Salmah Ismail, Lesley Maurice, Gwendelynne Tanil Bulan, Belinda Edwin, Cheah Yoke Kqueen and Son Radu. 2004. Isolation, antibiotic resistance and plasmid profiling of V. alginolyticus from cockles (Anadara granosa). Journal of Food Technology 2(1): 57-62.

Lesley, M., Son Radu, Kasing, A., Raha, A. R., Wong, C. M. V. L., Kqueen, C. Y. and Nishibuchi, M. 2003. Isolation and molecular characterization of Listeria monocytogenes and Listeria innocua from poultry meat. ASEAN Food Journal Vol. 12, No.2, 2003.

Endang Purwati, Son Radu, Ahmad Ismail, Cheah Yoke Kqueen and Lesley Maurice. 2003. Characterization of Listeria monocytogenes isolated from chicken meat: evidence of conjugal transfer of plasmid-mediated resistance to antibiotic. Journal of Animal and Veterinary Advances 2(4): 237-246, 2003.

L. M. Bilung, R. Son, J. Laurence, V. Vikneswaran, Y. K. Cheah, C. M. V. L. Wong, M. Nishibuchi. 2002. Vibrio parahaemolyticus from local cockles (Anadara granosa). 4th Ministry of Health Malaysia, Academy of Medicine of Malaysia Scientific Meeting, 2002. The Medical Journal of Malaysia, Vol. 57 (Supplement D), October 2002.

L. M. Bilung, R. Son, J. Laurence, V. Vikneswaran, Y. K. Cheah, C. M. V. L. Wong, M. Nishibuchi. 2002. Vibrio alginolyticus and Vibrio cholerae pathogens from cockles in three local markets. 4th Ministry of Health Malaysia, Academy of Medicine of Malaysia Scientific Meeting, 2002. The Medical Journal of Malaysia, Vol. 57 (Supplement D), October 2002.

J. Laurence, R. Son, A. A. Shaharudin, Y.K. Cheah, L. Samuel, L. Audrey, Lesley, Irene Lah. 2002. Correlation of air contamination and identification of pathogenic bacteria from environment. 4th Ministry of Health Malaysia, Academy of Medicine of Malaysia Scientific Meeting, 2002. The Medical Journal of Malaysia, Vol. 57 (Supplement D), October 2002.

L. Samuel, M. M. Marian, K. Apun, M. B. Lesley and R. Son 2010. Antibiotic resistance and genetic heterogeneity of Escherichia coli from cultured catfish and water environment. Malaysian Science and Technology Congress (MSTC), 9-11th November 2010, Crystal Crown, Petaling Jaya, Selangor.Pp 166 (abstract).

Kasing Apun, Lesley Maurice Bilung, Mohd. Tajuddin Abdullah, Kho Kai Ling, Chen Yik Ming, Adom Benjaman, Mustafa Abdul Rahman and Samuel Lihan. 2010. Eco-zoonoses project in Sarawak: hunting for enteric bacteria in wildlife. Proceedings of the 3rd Biotechnology Colloquium 2010, 24th-25th May 2010, Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak.

Adom Benjamen, Kasing Apun, Lesley Maurice Bilung and Mustafa Abdul Rahman. 2010. Isolation of Campylobacter spp. from migratory birds 57 and rodents in disturbed and undisturbed ecological habitats in Sarawak, Malaysia Proceedings of the 3rd Biotechnology Colloquium 2010, 24th-25th May 2010, Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak.

Chen Yik Ming, Kasing Apun, Mohd. Tajuddin Abdullah and Lesley Maurice Bilung. 2010. Prevalence of Shigella, Salmonella and Yersinia spp in disturbed 114 (human settlement) and natural (forest) habitats in Nanga Merit, Kapit, Sarawak. Proceedings of the 3rd Biotechnology Colloquium 2010, 24th-25th May 2010, Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak.

Micky Vincent, Lesley Maurice Bilung, Kasing Apun and Samuel Lihan. 2010. Detection of Vibrio parahaemolyticus in Raw Vegetables from Different Wet Markets in Kota Samarahan and Kuching, Sarawak. Proceedings of the International Conference on Food Research 2010, 22nd-24th November 2010, Marriott Putrajaya, Malaysia.

Lesley Maurice Bilung, Adom Benjamen, Kasing Apun, Samuel Lihan and Micky Vincent. 2010. Detection of Campylobacter spp. and Campylobacter jejuni in raw vegetables by using direct PCR method. Proceedings of the International Conference on Food Research 2010, 22nd-24th November 2010, Marriott Putrajaya, Malaysia.

Chen Yik Ming, Kasing Apun, Mohd. Tajuddin Abdullah, Lesley Maurice Bilung, Hashimatul Fatma Hashim, Kho Kai Ling and Adom Benjamen. 2010. Detection of virulence genes in Shigella, Salmonella and Yersinia spp. from wild animals in disturbed and natural habitats in Sarawak. Seminar of UNIMAS and FMNH. 17 December 2010.

Kho Kai Ling, Kasing Apun, Lesley Maurice Bilung, Hashimatul Fatma Hashim, Chong Yee Ling, Mohd. Tajuddin Abdullah, and Mustafa Abdul Rahman. 2010. Detection and characterization of Escherichia coli isolates by multiplex PCR and ERIC-PCR. Proceedings of the 3rd UNIMAS Colloquium 2010, 24th-25th May 2010, Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak.

Adom Benjamen, Kasing Apun, Lesley Maurice Bilung and Mustafa Abdul Rahman. 2010. Isolation of Campylobacter spp. from migratory birds and rodents in disturbed and undisturbed ecological habitats in Sarawak, Malaysia. UNIMAS-FMNH Seminar, 17th December 2010, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak.

Kasing Apun, Kho Kai Ling, Chong Yee Ling, Hashimatul Fatma Hashim, M.T. Abdullah, Lesley Maurice Bilung, and Mustafa Abdul Rahman. 2008. Enterobacteria isolated from birds and rodents from the urban forest in Sibu, Sarawak. Proceedings of the 10th Symposium Malaysian Society of Applied Biology, Kuching, 6-8 November 2008, p. 693-696.

Bilung, L.M., Vincent, M., Apun, K. and Tan, H.C. (2008) Molecular characterization of Vibrio alginolyticus isolated from Anadara granosa and environmental sources in Kuching-Samarahan District, Sarawak. Malaysian Science Congress Technology 2008. 16-17 December 2008. P. 64.

 

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