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Research Interest

My research interest involves the biochemistry, enzymology and molecular biology of microbial enzymes. In studying a particular enzyme of interest, molecular biology methods were employed as tools alongside protein chemistry studies to explore and understand the enzyme's function, regulation and degradation mechanisms.

Currently our research group works on the indigenous amylase/cellulase enzyme of Bacillus amyloliquefaciens UMAS 1002, which is capable of degrading sago pith waste. Genetic manipulation of the gene responsible for the amylolytic and cellulolytic properties has been successfully carried out and active recombinant protein was obtained through in vitro using the Escherichia coli expression system. We are hoping to be able to optimize the experimental conditions in order to increase the protein production. Achieving that, we will purify the native and recombinant crude enzymes to enable us to carry out in depth kinetic studies thus characterize the proteins.

The knowledge and information gained, would contribute profoundly in our quest to produce a robust and commercially available recombinant protein specifically for the biodegradation of sago industries residual waste.

1) Indigenous fungal isolates for bioremediation of pentachlorophenol (PCP), recalcitrant industrial pollutant.
2)Improvement of the CMS Agrotech Sdn Bhd Composting Technology for Production of Biofertilizer.
3) Production of amylase in solid substrate fermentation (SSF) using sago agro industrial residue as substrate by indigenous raw starch degrading fungi.
4) Isolation and characterization of marine bacteria involved in degradation of carbazole from coast of Sarawak.
5) Isolation, identification and characterisation of annomox
bacteria in Wastewater Treatment Plant in Kuching, Sarawak.
6) Isolation and characterization of novel marine bacteria involved in degradation of heterocyclic compound from coast of Sarawak.
7) Distribution and dynamics of indigenous hydrocarbon degrading fungi and their enzymes.

 

Publications

Journal Publications

Bioremediation of crude oil by different fungal genera. MF Kota, A Hussaini, A Zulkharnain, HA Roslan. Asian Journal of Plant Biology (e-ISSN 2289-5868) 2 (1), 16-23. 2014

Biodegradation and ligninolytic enzymes profiles of the newly synthesized organotin (IV)-treated non durable tropical wood species. MM Rahman, I Jusoh, A Husaini, I Abu Seman, NN Sing. Journal of Biochemical Technology 5 (3), 743-750. 2014

Accelerated production of white pepper using integrated mechanical and enzymatic solutions in an automated machine. MF Ashari, MD Ibrahim, A Husaini, A Zulkharnain. Key Engineering Materials 572, 304-307. 2014

Modelling row/column indicators for Boolean Matrices in DNA computing. N Rajaee, A Hussaini, A Zulkharnain, SMW Masra. International Journal of Advanced Technology and Science 1 (2), 34-40. 2014

Antimicrobial producing microbes isolated from soil samples collected from Nanga Merit Forest in Sarawak, Malaysian Borneo. S Lihan, CS Lin, I Ahmad, FM Sinang, NK Hua, AA Sallehin. European Journal of Experimental Biology 4 (1), 494-501. 2014.

Decolourisation of synthetic dyes by endophytic fungal flora isolated from Senduduk Plant (Melastoma malabathricum). NS Ngieng, A Zulkharnain, HA Roslan, A Husaini. ISRN Biotechnology 2013. 2013

Efficacy of novel organotin (IV) complexes on non-durable tropical wood against decay fungi. MM Rahman, I Jusoh, MA Affan, A Husaini, S Hamdan. European Journal of Wood and Wood Products 71 (4), 463-471. 2013

Preliminary Gene Characterization of α-Amylase from Bacillus amyloliquefaciens UMAS 1002. MSMAT HUSSIN, MH HUSSAIN, AASA HUSAINI, K BUJANG, DS AWG. Borneo Journal of Resource Science and Technology 3 (2), 36-39. 2013

Physico-Mechanical Properties of Tropical Wood Treated with Novel Organotin (IV) Complexes. MM Rahman, I Jusoh, MA Affan, A Husaini, S Hamdan, MS Islam. European Journal of Scientific Research 89 (4), 512-522. 2012

Molecular and biochemical characterization of antifungal antibiotic of Bacillus species Isolated from different pepper farms in Malaysia. YC Ann, AT Henry, AA Sallehin, HA Roslan. Journal of Pepper Industry 4 (2), 1-16. 2012

Molecular Phylogeny of Sarawak Green Sea Turtle (Chelonia mydas) inferred by the D-loop region and 16S rRNA gene. NK Yahya, R Hassan, A Husaini. Borneo Journal of Resource Science and Technology 2, 20-27. 2012

Lignocellulolytic enzymes produced by tropical white rot fungi during biopulping of Acacia mangium wood chips. A Husaini, FA Fisol, LC Yun, MH Hussain, HA Roslan. Journal of Biochemical Technology 3 (2), 245-250. 2011

Lignin biodegradation and ligninolytic enzyme studies during biopulping of Acacia mangium wood chips by tropical white rot fungi. CY Liew, A Husaini, H Hussain, S Muid, KC Liew, HA Roslan. World Journal of Microbiology and Biotechnology 27 (6), 1457-1468. 2011

XYLANASE GENE FROM A LOCALLY ISOLATED BACTERIUM. MH HUSSAIN, NFM CHONG, CSW CHAN, A SAFARINA, A HUSAINI. Journal of the Malaysian Society of Applied Biology 40 (1), 39-44. 2011

HOT PHENOL EXTRACTION OF TOTAL RNA FROM Thermoascus aurantiacus AND CHARACTERIZATION OF ITS THERMOSTABLE XYLANASE GENE. ANGC HUAP, AAS AWANG, HA ROSLAN. Borneo Journal of Resource Science and Technology 1, 55-58. 2010

Multiple forms of alcohol dehydrogenase (Adh) genes in sago palm: a preliminary study. HA Roslan, Y Sundaraj, AASA Husaini. Banwa: The Multidisciplinary Journal of UP Mindanao 5 (1), 30–41. 2009

Biodegradation of aliphatic hydrocarbon by indigenous fungi isolated from used motor oil contaminated sites. A Husaini, HA Roslan, KSY Hii, CH Ang. World Journal of Microbiology and Biotechnology 24 (12), 2789-2797. 2008

Malaysian fruit bats phylogeny inferred using ribosomal RNA. JJ Rovie-Ryan, AKH Guan, JV Kumaran, YB Esa, AA Sallehin. Pertanika J Trop Agric Sci 31 (1), 67-77. 2008

Enhancing a-amylase and cellulase in vivo enzyme expressions on sago pith residue using Bacilllus amyloliquefaciens UMAS 1002. F Khan, A Husaini. Biotechnology 5 (3), 391-403. 2006

 

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