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Research Interest

My major research interests cover two main disciplines: Bacterial Characterization and Biofuel Production.

1. Bacterial Characterization

My early research was on the molecular characterization and genetic analysis of Vibrio cholerae 01 from clinical samples. These included the studies to determine the genetic relatedness among isolates through PCR-based methods and the screening of specific genes (tcp, ace, ctx and zot) during cholera outbreaks. Other studies include the monitoring, isolation and molecular characteristics of  other important Vibrio spp. (V. cholerae non-01, V. parahaemolyticus, V. alginolyticus, V. vulnificus) from food and environmental sources in Sarawak. The more recent addition to my bacterial research is on the screening, phenotypic characterization and molecular ecology of potentially pathogenic Listeria monocytogenes and other opportunistic microbes that are present as indigenous microbiota (Staphylococcus spp., Streptococcus spp.) on the human body. One on-going exercise is to generate information on the emergence and prevalence of antimicrobial agents resistance, as a regular exercise to monitor the presence of multi-resistant strains. This is aimed to determine the pattern of resistance towards popular hospital prescribed antibiotics and antimicrobial agents (i.e. tetracycline and chloramphenicol). Analysis would also involve the use of conventional methods as well as using state-of the art technology involving molecular tools, such as plasmid DNA analysis and polymerase chain reaction based methods (RAPD, ERIC, BOX elements, Nested, Multiplex and specific gene monitoring). Ultimately, these efforts are hoped to cover major important naturally occurring food borne pathogens.

2. Biofuel Production

Production of liquid biofuel (bioethanol and biodiesel) is presently one of the hottest research topic globally. Thus, biofuel production is the latest addition to my research port-folio after completing my doctorate in Civil, Construction and Environmental Engineering, majoring in Biorenewable Resources Technology with special emphasis on Biofuel Production. My research covers both Second Generation Bioethanol and Third Generation Biofuel (Microbial Lipids) studies. For the production of biofuel via the two platforms, we are looking into the feasibility of production by using agricultural waste (i.e. sago waste, palm oil waste) and industrial waste. The method employed for bioethanol production will include the enzyme based simultaneous saccharification and fermentation (SSF) procedure.  In this part of the studies, several key parameters that will be investigated include pretreatment technologies and feedstocks testing. As for the production of microbial lipids, selected oleaginous fungi will be screened for suitability against several variables such as feedstock types, retention periods and other bio-chemo-physio parameters. The long term goal is to hit small scale production. We hope to partner up with local biofuel producers and foster strong collaboration ties in this area.


  1.       Son Radu, Micky Vincent, Kasing Apun, Raha AR, Patrick GB, Yuherman, Gulam R (2002) Molecular characterization of Vibrio cholerea 01 outbreak strains in Miri, Sarawak (Malaysia).  Acta Tropica 83: 169-176. (Q1, IF – 2.56). Link
  2.             Apun Kasing, Chang PP, Sim Edmund Ui Huang, Micky Vincent. (2005) Clonal diversity of Escherichia coli isolates from marketed beef in East Malaysia. World Journal of Microbiology Biotechnology 22: 661-667. (Q2, IF – 1.50). Link
  3.       Apun Kasing, Chong Yee Ling, Abdullah MT, Micky Vincent. 2008. Antimicrobial susceptibilities of Escherichia coli isolates from food, animals and wildlife animals in Sarawak, East Malaysia. Asian Journal of Animal and Veterinary Advances 3(6): 409-416. (Q2, IF – 0.84). Link
  4.       Micky Vincent, Anthony L. Pometto III and J. (Hans) van Leeuwen. 2011. Evaluation of potential fungal species for the in situ simultaneous saccharification and fermentation (SSF) of cellulosic material. Malaysian Journal of Microbiology 7(3): 129-138. (Q4, IF – 0.490). Link
  5.       Micky Vincent, Anthony L. Pometto III and J. (Hans) van Leeuwen. 2011. Simultaneous saccharification and fermentation of ground corn stover for the production of fuel ethanol using Phanerochaete chrysosporium, Gloeophyllum trabeum, Saccharomyces cerevisiae and Escherichia coli K011. Journal of Microbiology and  Biotechnology 21(7): 703–710. (Q2, IF – 1.19). Link
  6.             Lesley, M. B., Velnetti, L., Cheah, Y. K., Son, R., Kasing, A., Samuel, L., Micky, V. and Nishibuchi, M. 2011. Antibiotic resistance and plasmid profiling of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) at Tanjung Karang, Kuala Selangor. International Food Research Journal 18(3): 1183-1188. (Q2, IF – 0.880). Link
  7.       Micky Vincent, Anthony L. Pometto III, J. (Hans) van Leeuwen. 2014. Ethanol production via simultaneous saccharification and fermentation of sodium hydroxide treated corn stover using Phanerochaete chrysosporium and Gloeophyllum trabeum. Bioresource Technology (ISSN 09608524) 158: 1–6. (Q1, IF – 4.750). Link
  8.             Micky, V., Nur Quraitu’ Aini, T., Velnetti, L., Patricia Rowena, M. B., Christy, C. and Lesley Maurice, B. 2014. Development of a SYBR green based real-time polymerase chain reaction assay for specific detection and quantification of Vibrio parahaemolyticus from food and environmental samples. International Food Research Journal (ISSN 19854668) 21(3): 921-927. (Q2, IF – 0.880). Link
  9.             Micky V., Nor Hasni H. and Ennry E. 2014. Production of bioethanol from office waste via Simultaneous Saccharification and Fermentation. Bioremediation Science & Technology Research (e-ISSN 2289-5892). 2(1): 19-22. Link
  10.            Micky Vincent, Lai Lee San, Ng Lee Tze and Kasing Apun. 2014. Application of PCR-Based DNA fingerprinting techniques for the genetic differentiation of Vibrio cholerae Non-01/Non-0139 isolates from Kuching, Sarawak. Borneo Journal of Resource Science and Technology (ISSN 229-9769) 4(2): 21-34. Link
  11.            Micky Vincent, Noor Amalina Mahamad Norrezi, Kopli Bujang, Dayang Salwani Awang. Adeni. 2014. The effect of substrate concentration on enzymatic hydrolysis of selected food waste for glucose production. Bioremediation Science & Technology Research (e-ISSN 2289-5892) 2(2): 1-4. Link
  12.            Chan, C.S.W., Lau, S., Husaini, A.A.S.A., Zulkharnain, A., Apun, K., Bilung, L.M. and Vincent, M. 2014. Identification of methane-producing bacteria from palm oil mill sludge (POMS) with solid cud from ruminant stomach. Journal of Environmental Microbiology and Toxicology (e-ISSN 2289-5906). Journal of Biochemistry, Microbiology and Biotechnology. 1(1): 23-26. Link
  13.             Lesley, M.B., Yong, S.F., Velnetti, L., Pui, C.F., Micky, V., Apun, K. and Chong, Y.L. 2014. Incidence of Vibrio parahaemolyticus at estuarine region of Sg. Sarawak (Sarawak) influenced by physicochemical parameters. Bioremediation Science & Technology Research (e-ISSN 2289-5892). 2(1): 9-13. Link
  14.             Pui Chai Fung, Lesley Maurice Bilung, Nur Bainun Mohd Zin, Nurulhuda Najihah Binti Zainal Abidin, Micky Vincent, Kasing Apun. 2014. Risk of acquiring Vibrio parahaemolyticus in water and shrimp from an aquaculture farm. Kuroshio Science (ISSN 1882-823X) 8(1): 59-62. Link
  15.            Lesley Maurice Bilung, Micky Vincent, Kasing Apun and Mohd Tajuddin Abdullah. 2014. A study of bacterial species from wildlife and environmental samples from selected national parks in Sarawak, Malaysia.  Research Update. 10(1): 12.
  16.            Lesley Maurice Bilung, Yong Sy Fuh, Velnetti Linang, Adom Benjamin, Micky Vincent, Kasing Apun, Samuel Lihan and Chien Su Lin. 2014. Genomic diversity of cholera outbreak strains in East Malaysia. Malaysian Journal of Medicine and Health Sciences (ISSN 1675-8544) 10(2): 19-26. (Q4, IF – 0.10). Link
  17.            Micky Vincent, Berry Rence Anak Senawi, Ennry Esut, Norizawati Muhammad Nor and Dayang Salwani Awang Adeni. 2015. Sequential saccharification and simultaneous fermentation (SSSF) of sago hampas for the production of bioethanol. Accepted by Sains Malaysiana. (Q2, IF – 0.650).
  18.             Micky Vincent, Christy Chan Sien Wei and Kasing Apun. Molecular confirmation and characterization of Vibrio parahaemolyticus from retailed fish. Accepted by International Food Research Journal (ISSN 19854668) 21(3): 921-927. (Q2, IF – 0.880).
  19.            Christy Chan Sien Wei, Lau Seng, Awang Ahmad Sallehin Awang Hussaini, Azham Zulkharnain, Kasing Apun, Lesley Maurice Bilung and Micky Vincent. 2015. Biogas production from palm oil empty fruit bunch (PO-EFB) by co-digestion using palm oil mill effluent (POME) sludge and solid cud from ruminant stomach. Accepted by Journal of Oil Palm Research. (Q3, IF – 0.18).
  20.       Micky Vincent, Empina Jabang, Norizawati Muhamad Nur, Ennry Esut, Leo Bulin Unting, Dayang Salwani Awang Adeni. Simultaneous co-saccharification and fermentation of sago hampas for bioethanol production. Accepted by Agriculture Engineering International: CIGR. (Q3, IF – 0.40).


  • Grand Prize for University Research from the American Academy of Environmental Engineers (2009).
  • George Washington Carver (GWC) Award for Outstanding Student Achievement in Biorenewables  from the Bioeconomy Institute of  Iowa State University (2010).
  • Bronze Medal from UNIMAS during the 2011 Research and Development Expo for work on “Cheap Biodiesel from Spent Cooking Oil”.
  • Bronze Medal from UNIMAS during the 2011 Research and Development Expo for work on “Bioethanol from Office Waste for Fuel”.

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